A new size-exclusion chromatography method for fast rapeseed albumin and globulin quantification
نویسندگان
چکیده
منابع مشابه
A hemagglutinin quantification method for development of an influenza pandemic vaccine using size exclusion high performance liquid chromatography.
Single radial immunodiffusion (SRID) assay requires a reference antigen and an antibody to the hemagglutinin (HA) of an influenza vaccine. As it takes 2‑3 months to develop the reference antigen, vaccine development is delayed in cases of an influenza pandemic. In the present study, the measurement of the HA content of influenza vaccines was assessed using size exclusion high performance liquid...
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In the characterization of polymers, size-exclusion chromatography (SEC) has become a standard technique for determining molar mass averages andmolar mass distributions (MMD) of polymers. The principle of SEC is easily understood: owing to limited accessability of the pore volume within the particles of the column packing, polymer molecules are separated according to their hydrodynamic volumes,...
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The shortening of the 3'-end poly(A) tail, also called deadenylation, is crucial to the regulation of mRNA processing, transportation, translation and degradation. The deadenylation process is achieved by deadenylases, which specifically catalyze the removal of the poly(A) tail at the 3'-end of eukaryotic mRNAs and release 5'-AMP as the product. To achieve their physiological functions, all dea...
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A combination of high performance size exclusion chromatography with organic carbon detector and ultraviolet detector coupled with peak-fitting technique and fluorescence excitation-emission matrix spectrometry applied fluorescence regional integration method was conducted to determine the characteristics of organic matter during nitrification. The batch scale of bionet nitrification without or...
متن کاملComparison between immunoturbidimetry, size-exclusion chromatography, and LC-MS to quantify urinary albumin.
BACKGROUND The accurate and precise measurement of urinary albumin is critical, since even minor increases are diagnostically sensitive indicators of renal disease, cardiovascular events, and risk for death. To gain insights into potential measurement biases, we systematically compared urine albumin measurements performed by LC-MS, a clinically available immunoturbidimetric assay, and size-excl...
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ژورنال
عنوان ژورنال: Food Chemistry
سال: 2019
ISSN: 0308-8146
DOI: 10.1016/j.foodchem.2019.01.209